A potential MRI agent and an anticancer drug encapsulated within CPMV virus-like particles

Alaa A A Aljabali; Lorca Alzoubi; Yassmen Hamzat; Alaa Alqudah; Mohammad A. Obeid; Mazhar S. Al Zoubi; Raed M Ennab; Walhan Alshaer; Khaled Albatayneh; Bahaa Al‑trad; Dana A. Alqudah; Dinesh Kumar Chellappan; Gaurav Gupta; Murtaza M. Tambuwala; Dua Kamal; David J Evans

doi:10.2174/1386207323666200914110012

A potential MRI agent and an anticancer drug encapsulated within CPMV virus-like particles

Alaa A A Aljabali, Lorca Alzoubi, Yassmen Hamzat, Alaa Alqudah, Mohammad A. Obeid, Mazhar S. Al Zoubi, Raed M Ennab, Walhan Alshaer, Khaled Albatayneh, Bahaa Al‑trad, Dana A. Alqudah, Dinesh Kumar Chellappan, Gaurav Gupta, Murtaza M. Tambuwala, Dua Kamal, David J Evans

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Abstract

Background: Virus nanoparticles have been extensively studied over the past decades for theranostics applications. Viruses are well-characterized, naturally occurring nanoparticles that can be produced in high quantity with a high degree of similarity in both structure and composition. Objectives: The plant virus Cowpea Mosaic Virus (CPMV) has been innovatively used as a nanoscaffold. Utilization of the internal cavity of empty Virus-Like Particles (VLPs) for the inclusion of therapeutics within the capsid has opened many opportunities in drug delivery and imaging applications. Methods: The encapsidation of magnetic materials and anticancer drugs was achieved. SuperscriptCPMV denotes molecules attached to the external surface of CPMV and CPMVSubscript denotes molecules within the interior of the capsid. Results: Here, the generation of novel VLPs incorporating iron-platinum nanoparticles TCPMVFePt and cisplatin (Cis) (TCPMVCis) is reported. TCPMVCis exhibited a cytotoxic IC50 of TCPMVCis on both A549 and MDA-MB-231 cell lines of 1.8 μM and 3.9 μM, respectively after 72 hours of incubation. The TCPMVFePt were prepared as potential MRI contrast agents. Conclusions: Cisplatin loaded VLP (TCPMVCis) is shown to enhance cisplatin cytotoxicity in cancer cell lines with its potency increased by 2.3-folds.

Original language	English
Pages (from-to)	1557-1571
Number of pages	15
Journal	Combinatorial Chemistry & High Throughput Screening
Volume	24
Issue number	10
Early online date	14 Sept 2020
DOIs	https://doi.org/10.2174/1386207323666200914110012
Publication status	Published online - 14 Sept 2020

Keywords

Organic Chemistry
Computer Science Applications
Drug Discovery
General Medicine

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.2174/1386207323666200914110012

Aljabali MS_PublishedAuthor Accepted Manuscript, 4.55 MB

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Aljabali, A. A. A., Alzoubi, L., Hamzat, Y., Alqudah, A., Obeid, M. A., Al Zoubi, M. S., Ennab, R. M., Alshaer, W., Albatayneh, K., Al‑trad, B., Alqudah, D. A., Chellappan, D. K., Gupta, G., Tambuwala, M. M., Kamal, D., & Evans, D. J. (2020). A potential MRI agent and an anticancer drug encapsulated within CPMV virus-like particles. Combinatorial Chemistry & High Throughput Screening, 24(10), 1557-1571. Advance online publication. https://doi.org/10.2174/1386207323666200914110012

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title = "A potential MRI agent and an anticancer drug encapsulated within CPMV virus-like particles",

abstract = "Background: Virus nanoparticles have been extensively studied over the past decades for theranostics applications. Viruses are well-characterized, naturally occurring nanoparticles that can be produced in high quantity with a high degree of similarity in both structure and composition. Objectives: The plant virus Cowpea Mosaic Virus (CPMV) has been innovatively used as a nanoscaffold. Utilization of the internal cavity of empty Virus-Like Particles (VLPs) for the inclusion of therapeutics within the capsid has opened many opportunities in drug delivery and imaging applications. Methods: The encapsidation of magnetic materials and anticancer drugs was achieved. SuperscriptCPMV denotes molecules attached to the external surface of CPMV and CPMVSubscript denotes molecules within the interior of the capsid. Results: Here, the generation of novel VLPs incorporating iron-platinum nanoparticles TCPMVFePt and cisplatin (Cis) (TCPMVCis) is reported. TCPMVCis exhibited a cytotoxic IC50 of TCPMVCis on both A549 and MDA-MB-231 cell lines of 1.8 μM and 3.9 μM, respectively after 72 hours of incubation. The TCPMVFePt were prepared as potential MRI contrast agents. Conclusions: Cisplatin loaded VLP (TCPMVCis) is shown to enhance cisplatin cytotoxicity in cancer cell lines with its potency increased by 2.3-folds.",

keywords = "Organic Chemistry, Computer Science Applications, Drug Discovery, General Medicine",

author = "Aljabali, {Alaa A A} and Lorca Alzoubi and Yassmen Hamzat and Alaa Alqudah and Obeid, {Mohammad A.} and {Al Zoubi}, {Mazhar S.} and Ennab, {Raed M} and Walhan Alshaer and Khaled Albatayneh and Bahaa Al‑trad and Alqudah, {Dana A.} and Chellappan, {Dinesh Kumar} and Gaurav Gupta and Tambuwala, {Murtaza M.} and Dua Kamal and Evans, {David J}",

year = "2020",

month = sep,

day = "14",

doi = "10.2174/1386207323666200914110012",

language = "English",

volume = "24",

pages = "1557--1571",

journal = "Combinatorial Chemistry & High Throughput Screening",

issn = "1386-2073",

publisher = "Bentham Science Publishers B.V.",

number = "10",

}

Aljabali, AAA, Alzoubi, L, Hamzat, Y, Alqudah, A, Obeid, MA, Al Zoubi, MS, Ennab, RM, Alshaer, W, Albatayneh, K, Al‑trad, B, Alqudah, DA, Chellappan, DK, Gupta, G, Tambuwala, MM, Kamal, D & Evans, DJ 2020, 'A potential MRI agent and an anticancer drug encapsulated within CPMV virus-like particles', Combinatorial Chemistry & High Throughput Screening, vol. 24, no. 10, pp. 1557-1571. https://doi.org/10.2174/1386207323666200914110012

TY - JOUR

T1 - A potential MRI agent and an anticancer drug encapsulated within CPMV virus-like particles

AU - Aljabali, Alaa A A

AU - Alzoubi, Lorca

AU - Hamzat, Yassmen

AU - Alqudah, Alaa

AU - Obeid, Mohammad A.

AU - Al Zoubi, Mazhar S.

AU - Ennab, Raed M

AU - Alshaer, Walhan

AU - Albatayneh, Khaled

AU - Al‑trad, Bahaa

AU - Alqudah, Dana A.

AU - Chellappan, Dinesh Kumar

AU - Gupta, Gaurav

AU - Tambuwala, Murtaza M.

AU - Kamal, Dua

AU - Evans, David J

PY - 2020/9/14

Y1 - 2020/9/14

N2 - Background: Virus nanoparticles have been extensively studied over the past decades for theranostics applications. Viruses are well-characterized, naturally occurring nanoparticles that can be produced in high quantity with a high degree of similarity in both structure and composition. Objectives: The plant virus Cowpea Mosaic Virus (CPMV) has been innovatively used as a nanoscaffold. Utilization of the internal cavity of empty Virus-Like Particles (VLPs) for the inclusion of therapeutics within the capsid has opened many opportunities in drug delivery and imaging applications. Methods: The encapsidation of magnetic materials and anticancer drugs was achieved. SuperscriptCPMV denotes molecules attached to the external surface of CPMV and CPMVSubscript denotes molecules within the interior of the capsid. Results: Here, the generation of novel VLPs incorporating iron-platinum nanoparticles TCPMVFePt and cisplatin (Cis) (TCPMVCis) is reported. TCPMVCis exhibited a cytotoxic IC50 of TCPMVCis on both A549 and MDA-MB-231 cell lines of 1.8 μM and 3.9 μM, respectively after 72 hours of incubation. The TCPMVFePt were prepared as potential MRI contrast agents. Conclusions: Cisplatin loaded VLP (TCPMVCis) is shown to enhance cisplatin cytotoxicity in cancer cell lines with its potency increased by 2.3-folds.

AB - Background: Virus nanoparticles have been extensively studied over the past decades for theranostics applications. Viruses are well-characterized, naturally occurring nanoparticles that can be produced in high quantity with a high degree of similarity in both structure and composition. Objectives: The plant virus Cowpea Mosaic Virus (CPMV) has been innovatively used as a nanoscaffold. Utilization of the internal cavity of empty Virus-Like Particles (VLPs) for the inclusion of therapeutics within the capsid has opened many opportunities in drug delivery and imaging applications. Methods: The encapsidation of magnetic materials and anticancer drugs was achieved. SuperscriptCPMV denotes molecules attached to the external surface of CPMV and CPMVSubscript denotes molecules within the interior of the capsid. Results: Here, the generation of novel VLPs incorporating iron-platinum nanoparticles TCPMVFePt and cisplatin (Cis) (TCPMVCis) is reported. TCPMVCis exhibited a cytotoxic IC50 of TCPMVCis on both A549 and MDA-MB-231 cell lines of 1.8 μM and 3.9 μM, respectively after 72 hours of incubation. The TCPMVFePt were prepared as potential MRI contrast agents. Conclusions: Cisplatin loaded VLP (TCPMVCis) is shown to enhance cisplatin cytotoxicity in cancer cell lines with its potency increased by 2.3-folds.

KW - Organic Chemistry

KW - Computer Science Applications

KW - Drug Discovery

KW - General Medicine

U2 - 10.2174/1386207323666200914110012

DO - 10.2174/1386207323666200914110012

M3 - Article

C2 - 32928083

SN - 1386-2073

VL - 24

SP - 1557

EP - 1571

JO - Combinatorial Chemistry & High Throughput Screening

JF - Combinatorial Chemistry & High Throughput Screening

IS - 10

ER -

A potential MRI agent and an anticancer drug encapsulated within CPMV virus-like particles

Abstract

Keywords

UN SDGs

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