An electrochemical adaptation of Ellman's test

O Nekrassova, PC White, S Threlfell, G Hignett, AJ Wain, NS Lawrence, J Davis, RG Compton

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    37 Citations (Scopus)

    Abstract

    The electrochemically initiated reaction of thiols with N,N-diethyl-p-phenylenediamine has been coupled with an existing colorimetric sensing reaction developed by Ellman as a means of providing an electrochemical adaptation of the latter whereby the total thiol species present in a sample can be determined. The detection methodology has been proven to be robust with a linear range for cysteine from 2-120 muM, a limit of detection of 1.17 muM and has shown selectivity against a wide range of potential interferences. The efficiency of the methodology has been examined in the determination and recovery of thiol species in three growth tissue media, which contain a number of common biological interferences.
    Original languageEnglish
    Pages (from-to)797-802
    JournalANALYST
    Volume127
    Issue number6
    DOIs
    Publication statusPublished - Jun 2002

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