Antiproliferative effects of boswellic acids-loaded chitosan nanoparticles on human lung cancer cell line A549

Neeta Solanki, Meenu Mehta, Dinesh Kumar Chellappan, Gaurav Gupta, Nicole G. Hansbro, Murtaza M. Tambuwala, Alaa Aa Aljabali, Keshav Raj Paudel, Gang Liu, Saurabh Satija, Philip M. Hansbro, Kamal Dua, Harish Dureja

Research output: Contribution to journalArticlepeer-review

4 Citations (Scopus)

Abstract

Aim: In the present study boswellic acids-loaded chitosan nanoparticles were synthesized using ionic gelation technique. The influence of independent variables were studied and optimized on dependent variables using central composite design. Methodology & results: The designed nanoparticles were observed spherical in shape with an average size of 67.5-187.2 nm and have also shown an excellent entrapment efficiency (80.06 ± 0.48). The cytotoxicity assay revealed enhanced cytotoxicity for drug-loaded nanoparticles in contrast to the free drug having an IC50 value of 17.29 and 29.59 μM, respectively. Flow cytometry confirmed that treatment of cells with 40 μg/ml had arrested 22.75 ± 0.3% at SubG0 phase of the cell cycle when compared with untreated A459 cells. The observed results justified the boswellic acids-loaded chitosan nanoparticles were effective due to greater cellular uptake, sustained intercellular drug retention and enhanced antiproliferative effect by inducing apoptosis.

Original languageEnglish
Pages (from-to)2019-2034
Number of pages16
JournalFuture Medicinal Chemistry
Volume12
Issue number22
Early online date30 Oct 2020
DOIs
Publication statusPublished - 30 Nov 2020

Bibliographical note

Funding Information:
The authors are thankful to the Graduate School of Health, University of Technology Sydney, Australia. M Mehta is supported by the University of Technology Sydney International Research Training Program Scholarship (IRTP) and International Research Training Program Scholarship (IRTP). K Dua is supported by a project grant from Rebecca L Cooper Medical Research Foundation and Sydney Partnership for Health, Education, Research and Enterprise (SPHERE) for the TRIPLE I CAG Secondment/Exchange grant. The authors have no other relevant affiliations or financial involvement with any organization or entity with a financial interest in or financial conflict with the subject matter or materials discussed in the manuscript apart from those disclosed. No writing assistance was utilized in the production of this manuscript.

Publisher Copyright:
© 2020 Future Medicine Ltd.. All rights reserved.

Copyright:
Copyright 2020 Elsevier B.V., All rights reserved.

Keywords

  • bioavailability
  • boswellic acids
  • chitosan
  • lung cancer
  • nanoparticles

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