Correlation between microbial protein thermostability and resistance to denaturation in aqueous-organic solvent 2-phase systems

RK Owusu, DA Cowan

Research output: Contribution to journalArticlepeer-review

86 Citations (Scopus)

Abstract

The thermostability of cell-free protein extracts (from 10 microorganisms grown at 37–84°C) and of 10 fully or partially purified neutral proteases was determined in aqueous solution (Tris-HCl buffer 0.1 m. pH 7.0) using protein solubility and enzyme activity loss, respectively, as the criteria of denaturation. The stability of the protein extracts and purified proteins was also determined in an aqueous-water immiscible organic solvent two-phase system using the same criteria. There was a strong positive correlation between microorganism growth temperature and the thermostability of protein extracts in both aqueous and aqueous:organic two-phase systems. A correlation also existed between protease thermostability and the stability in the aqueous:organic two-phase system. Protein stability was higher in those aqueous:organic two-phase systems with a more hydrophobic organic phase. However, for pairs of organic solvents of similar hydrophobicity, the aqueous:organic two-phase system with the higher rated interfacial tension was more denaturing. Protein extract stability was sensitive to both aqueous:gas and aqueous:organic interface size depending on the nature of the organic phase.
Original languageEnglish
Pages (from-to)568-574
JournalEnzyme and Microbial Technology
Volume11
Issue number9
DOIs
Publication statusPublished - 1 Sep 1989

Bibliographical note

Reference text: 1. AHERN TJ
THE MECHANISM OF IRREVERSIBLE ENZYME INACTIVATION AT 100-DEGREES-C
SCIENCE 228 : 1280 1985
2. AMELUNXEN RE
MECHANISMS OF THERMOPHILY
CRC CRITICAL REVIEWS IN MICROBIOLOGY 6 : 343 1978
3. ANTONINI E
ENZYME CATALYZED-REACTIONS IN WATER-ORGANIC SOLVENT 2-PHASE SYSTEMS
ENZYME AND MICROBIAL TECHNOLOGY 3 : 291 1981
4. BRINK LES
OPTIMIZATION OF ORGANIC-SOLVENT IN MULTIPHASE BIOCATALYSIS
BIOTECHNOLOGY AND BIOENGINEERING 27 : 1258 1985
5. BROCK TD
LIFE AT HIGH-TEMPERATURES
SCIENCE 230 : 132 1985
6. BUTLER LG
ENZYMES IN NON-AQUEOUS SOLVENTS
ENZYME AND MICROBIAL TECHNOLOGY 1 : 253 1979
7. CARREA G
BIOCATALYSIS IN WATER-ORGANIC SOLVENT 2-PHASE SYSTEMS
TRENDS IN BIOTECHNOLOGY 2 : 102 1984
8. CLAUS D
DTSCH SAMMLUNG MIKRO : 1983
9. COWAN DA
PURIFICATION AND SOME PROPERTIES OF AN EXTRACELLULAR PROTEASE (CALDOLYSIN) FROM AN EXTREME THERMOPHILE
BIOCHIMICA ET BIOPHYSICA ACTA 705 : 293 1982
10. DANIEL RM
A CORRELATION BETWEEN PROTEIN THERMOSTABILITY AND RESISTANCE TO PROTEOLYSIS
BIOCHEMICAL JOURNAL 207 : 641 1982
11. DAVIES JT
INTERFACIAL PHENOMEN : 1 1961
12. DONALDSON TL
KINETICS OF PROTEIN DENATURATION AT GAS-LIQUID INTERFACES
JOURNAL OF COLLOID AND INTERFACE SCIENCE 74 : 441 1980
13. FRANKS F
CRC CRIT REV BIOCHEM 3 : 165 1975
14. FRENDSDORFF HK
THE KINETICS OF PROTEIN DENATURATION .4. THE VISCOSITY AND GELATION OF UREA SOLUTIONS OF OVALBUMIN
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY 75 : 5157 1953
15. FUKUSHIMA D
DENATURATION OF SOYBEAN PROTEINS BY ORGANIC SOLVENTS
CEREAL CHEMISTRY 46 : 156 1969
16. GRAHAM DE
PROTEINS AT LIQUID INTERFACES .3. MOLECULAR-STRUCTURES OF ADSORBED FILMS
JOURNAL OF COLLOID AND INTERFACE SCIENCE 70 : 427 1979
17. HICKEY CW
ELECTRON-TRANSPORT SYSTEM OF AN EXTREMELY THERMOPHILIC BACTERIUM
JOURNAL OF GENERAL MICROBIOLOGY 114 : 195 1979
18. HOLME J
THERMAL DENATURATION AND AGGREGATION OF OVALBUMIN
JOURNAL OF PHYSICAL CHEMISTRY 67 : 782 1963
19. HORBETT TA
PROTEINS INTERFACES : 1 1987
20. HUDSON JA
ISOLATION AND CHARACTERIZATION OF A NEW CALDOACTIVE FILAMENTOUS BACTERIUM
FEMS MICROBIOLOGY LETTERS 22 : 149 1984
21. JAMES LK
ADSORPTION OF ENZYMES AT INTERFACES - FILM FORMATION AND EFFECT ON ACTIVITY
ADVANCES IN ENZYMOLOGY AND RELATED AREAS OF MOLECULAR BIOLOGY 28 : 1 1966
22. JOLY M
PHYSICO CHEM APPROAC : 1965
23. KHMELNITSKY YL
DETERGENTLESS MICROEMULSIONS - A NEW MICROHETEROGENEOUS MEDIUM FOR ENZYMATIC-REACTION
ANNALS OF THE NEW YORK ACADEMY OF SCIENCES 501 : 161 1987
24. KLIBANOV AM
STABILIZATION OF ENZYMES AGAINST THERMAL INACTIVATION
ADVANCES IN APPLIED MICROBIOLOGY 29 : 1 1983
25. KOFFLER H
THE RELATIVE THERMOSTABILITY OF CYTOPLASMIC PROTEINS FROM THERMOPHILIC BACTERIA
ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS 67 : 249 1957
26. LAANE C
BIOCATALYSIS ORGANIC : 65 1987
27. LILLY MD
2-LIQUID-PHASE BIOCATALYTIC REACTIONS
JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY 32 : 162 1982
28. MOZHAEV VV
STRUCTURE STABILITY RELATIONSHIPS IN PROTEINS - NEW APPROACHES TO STABILIZING ENZYMES
ENZYME AND MICROBIAL TECHNOLOGY 6 : 50 1984
29. OSHIMA T
ENZYME ENG 4 : 41 1978
30. PETERSON GL
SIMPLIFICATION OF PROTEIN ASSAY METHOD OF LOWRY ET AL - WHICH IS MORE GENERALLY APPLICABLE
ANALYTICAL BIOCHEMISTRY 83 : 346 1977


31. ROTHERN A
ADV PROTEIN CHEM 3 : 123 1948
32. SCHELLMAN JA
SOLVENT DENATURATION
BIOPOLYMERS 17 : 1305 1978
33. SCHRIER EE
EFFECT OF AQUEOUS ALCOHOL SOLUTIONS ON THERMAL TRANSITION OF RIBONUCLEASE
JOURNAL OF PHYSICAL CHEMISTRY 69 : 298 1965
34. SINGER SJ
THE PROPERTIES OF PROTEINS IN NONAQUEOUS SOLVENTS
ADVANCES IN PROTEIN CHEMISTRY 17 : 1 1962
35. SODA K
TRENDS BIOCHEM SCI 8 : 428 1987
36. SOKAL RR
INTRO BIOSTATISTICS : 260 1973
37. SUZUKI C
GELATION OF OVALBUMIN SOLUTIONS BY HIGH PRESSURES
ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS 102 : 367 1963
38. TANFORD C
ADV PROTEIN CHEM 23 : 121 1968
39. ZAKS A
J BIOL CHEM 236 : 1988
40. ZALE SE
WHY DOES RIBONUCLEASE IRREVERSIBLY INACTIVATE AT HIGH-TEMPERATURES
BIOCHEMISTRY 25 : 5432 1986
41. ZALE SE
ON THE ROLE OF REVERSIBLE DENATURATION (UNFOLDING) IN THE IRREVERSIBLE THERMAL INACTIVATION OF ENZYMES
BIOTECHNOLOGY AND BIOENGINEERING 25 : 2221 1983

Keywords

  • Thermophilic proteins
  • protein thermostability: protein stability
  • aqueous:organic solvent two-phase systems

Fingerprint

Dive into the research topics of 'Correlation between microbial protein thermostability and resistance to denaturation in aqueous-organic solvent 2-phase systems'. Together they form a unique fingerprint.

Cite this