Abstract
Giardia duodenalis and Cryptosporidium spp. are two of the most prominent etiological agents of waterborne diseases. Therefore, efficient and affordable methodologies for identifying and quantifying these parasites in water are increasingly necessary. USEPA Method 1623.1 is a widely used and validated protocol for detecting these parasites in water samples. It consists of a concentration step, followed by parasite purification and visualization by immunofluorescence microscopy. Although efficient, this method has a high cost particularly due to the immunomagnetic separation (IMS) step, which is most needed with complex and highly contaminated samples. Based on this, the present study aimed to determine whether it is possible to maintain the efficiency of Method 1623.1 whilst reducing the amount of beads per reaction, using as matrix the challenge water recommended by the World Health Organization. As for Giardia cysts, a satisfactory recovery efficiency (RE) was obtained using 50% less IMS beads. This was evaluated both with a commercial cyst suspension (56.1% recovery) and an analytical quality assessment (47.5% recovery). Although RE rates obtained for C. parvum did not meet Method 1623.1 criteria in any of the experimental conditions tested, results presented in this paper indicated the relevance of the described adaptations, even in challenge water.
Original language | English |
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Article number | jwh2021005 |
Pages (from-to) | 1-12 |
Number of pages | 12 |
Journal | Journal of Water and Health |
Early online date | 31 Mar 2021 |
DOIs | |
Publication status | E-pub ahead of print - 31 Mar 2021 |
Keywords
- Cryptosporidium spp. oocysts
- Giardia spp. cysts
- low-cost recovery methods
- parasitic protozoa
- recovery efficiency