The present paper examines how the sex-role orientation of the nurse can affect both positive and negative assertion. The Bem Sex-Role Inventory (BRSI) and the Assertion Inventory (AI) were used to collect self-reported data from a convenience sample of 94 subjects. Behavioral data were obtained by directly sampling 50 nurses’ responses within role-play situations. Contrary to earlier studies, the findings of the investigation indicated that there was no significant relationship between assertion measurements and sex-role orientation. Following a discussion of the findings, recommendations relating to future research are made and the limitations of the study are identified.