An enthalpimetric determination of urea using immobilized urease in a flow system is described. Sensitivity of analysis is related to the mole reaction enthalpy (dHR). This is estimated as (61 ±3 kJ mol−1 and 20 ±1 kJ mol−1 in sodium phosphate and tris-HCl buffer respectively. Factors affecting the minimum amount of urea detected, linearity range and rate of analysis are discussed.
|Publication status||Published - 15 Feb 1987|