Evaluation of bactericidal and anti-biofilm properties of a novel surface-active organosilane biocide against healthcare associated pathogens and Pseudomonas aeruginosa biolfilm

Jason Murray, Tendai Muruko, Chris IR Gill, Patricia Kearney, David Farren, Michael G Scott, Geoff McMullan, Nigel G Ternan

Research output: Contribution to journalArticlepeer-review

4 Citations (Scopus)
25 Downloads (Pure)


Healthcare acquired infections (HAI) pose a great threat in hospital settings and environmental contamination can be attributed to the spread of these. De-contamination and, significantly, prevention of re-contamination of the environment could help in preventing/reducing this threat. Goldshield (GS5) is a novel organosilane biocide marketed as a single application product with residual biocidal activity. We tested the hypothesis that GS5 could provide longer-term residual antimicrobial activity than existing disinfectants once applied to surfaces. Thus, the residual bactericidal properties of GS5, Actichlor and Distel against repeated challenge with Staphylococcus aureus ATCC43300 were tested, and showed that GS5 alone exhibited longer-term bactericidal activity for up to 6 days on 316I stainless steel surfaces. Having established efficacy against S. aureus, we tested GS5 against common healthcare acquired pathogens, and demonstrated that, on average, a 1 log10 bactericidal effect was exhibited by GS5 treated surfaces, although biocidal activity varied depending upon the surface type and the species of bacteria. The ability of GS5 to prevent Pseudomonas aeruginosa biofilm formation was measured in standard microtitre plate assays, where it had no significant effect on either biofilm formation or development. Taken together the data suggests that GS5 treatment of surfaces may be a useful means to reducing bacterial contamination in the context of infection control practices.
Original languageEnglish
JournalPLoS ONE
Issue number8
Publication statusPublished - 7 Aug 2017

Bibliographical note

Reference text: 1. Zingg W, Holmes A, Dettenkofer M, Goetting T, Secci F, Clack L, et al. Hospital organisation, management, and structure for prevention of health-care-associated infection: a systematic review and expert consensus. Lancet. Infect. Dis. 2015;15:212–224
2. Zimlichman E, Henderson D, Tamir O, Franz C, Song P, Yamin CK, et al. . Health care-associated infections: a meta-analysis of costs and financial impact on the US health care system. JAMA Intern Med. 2013;173:2039–2046.
3. van Kleef E, Robotham J V, Jit M, Deeny SR, Edmunds WJ. Modelling the transmission of healthcare associated infections: a systematic review. BMC Infect. Dis. 2013;13:294
4. Wagenvoort JH, Sluijsmans W, Penders RJ. Better environmental survival of outbreak vs. sporadic MRSA isolates. J. Hosp. Infect. 2000;45:231–234
5. Otter JA, Yezli S, Salkeld JAG, French GL. Evidence that contaminated surfaces contribute to the transmission of hospital pathogens and an overview of strategies to address contaminated surfaces in hospital settings. Am. J. Infect. Control. 2013;41:S6–11
6. Wagenvoort JHT, De Brauwer EIGB, Penders RJR, Willems RJ, Top J, Bonten MJ. Environmental survival of vancomycin-resistant Enterococcus faecium. J. Hosp. Infect. 2011;77:282–283
7. Thomas E, Bémer P, Eckert C, Guillouzouic A, Orain J, Corvec S, et al. Clostridium difficile infections: analysis of recurrence in an area with low prevalence of 027 strain. J. Hosp. Infect. 2016.
8. Abdallah M, Benoliel C, Drider D, Dhulster P, Chihib N-E. Biofilm formation and persistence on abiotic surfaces in the context of food and medical environments. Arch. Microbiol., vol. 196, no. 7, pp. 453–472, Jul. 2014.
9. Walker JT, Jhutty A, Parks S, Willis C, Copley V, Turton JF, et al. Investigation of healthcare-acquired infections associated with Pseudomonas aeruginosa biofilms in taps in neonatal units in Northern Ireland. J. Hosp. Infect. 2014;86:16–23
10. Hota B. Contamination, disinfection, and cross-colonization: are hospital surfaces reservoirs for nosocomial infection? Clin. Infect. Dis. 2004;39:1182–1189
11. French GL, Otter JA, Shannon KP, Adams NMT, Watling D, Parks MJ. Tackling contamination of the hospital environment by methicillin-resistant Staphylococcus aureus (MRSA): a comparison between conventional terminal cleaning and hydrogen peroxide vapour decontamination. J. Hosp. Infect. 2004;57:31–33
12. Beggs C, Knibbs LD, Johnson GR, Morawska L. Environmental contamination and hospital-acquired infection: factors that are easily overlooked. Indoor Air. 2015;25:462–474
13. Ramphal L, Suzuki S, McCracken IM, Addai A. Improving hospital staff compliance with environmental cleaning behavior. Proc. (Bayl. Univ. Med. Cent). 2014;27:88–91
14. Hardy KJ, Oppenheim BA, Gossain S, Gao F, Hawkey PM. A study of the relationship between environmental contamination with methicillin-resistant Staphylococcus aureus (MRSA) and patients’ acquisition of MRSA. Infect. Control Hosp. Epidemiol. 2006;27:127–132
15. Abreu AC, Tavares RR, Borges A, Mergulhão F, Simões M. Current and emergent strategies for disinfection of hospital environments. J. Antimicrob. Chemother. 2013;68:2718–2732
16. Aldeyab MA, Mcelnay JC, Elshibly SM, Hughes CM, Mcdowell DA, McMahon MAS, et al. Evaluation of the Efficacy of a Conventional Cleaning Regimen in Removing Methicillin ‐ Resistant Staphylococcus aureus From Contaminated Surfaces in an Intensive Care Unit. Infect Control Hosp Epidemiol. 2009;30(3):304-306.
17. Muller MP, MacDougall C, Lim M. Antimicrobial surfaces to prevent healthcare-associated infections: a systematic review. J. Hosp. Infect. 2015;92:7–13
18. Diseases and Organisms in Healthcare Settings. U.S. Centers for Disease Control and Prevention Website. http://www.cdc.gov/HAI/organisms/organisms.html. Published 2014. Accessed 08 March 2016.
19. Altaf M, Miller CH, Bellows DS, O’Toole R. Evaluation of the Mycobacterium smegmatis and BCG models for the discovery of Mycobacterium tuberculosis inhibitors. Tuberculosis (Edinb). 2010;90:333–337
20. Baxa D, Shetron-Rama L, Golembieski M, Golembieski M, Jain S, Gordon M, et al. In vitro evaluation of a novel process for reducing bacterial contamination of environmental surfaces. Am. J. Infect. Control. 2011;39:483–487
21. Djordjevic D, Wiedmann M, McLandsborough LA. Microtiter Plate Assay for Assessment of Listeria monocytogenes Biofilm Formation. Appl. Environ. Microbiol. 2002;68:2950–2958
22. O’Toole, GA. Microtitre Dish Biofilm Formation Assay. JoVE. 2011;47:2437.
23. Shen Y, Köller T, Kreikemeyer B, Nelson DC. Rapid degradation of Streptococcus pyogenes biofilms by PlyC, a bacteriophage-encoded endolysin. J. Antimicrob. Chemother. 2013;68:1818–1824
24. Webb JS, Thompson LS, James S, Charlton T, Tolker-Nielsen T, Koch B, et al. Cell Death in Pseudomonas aeruginosa Biofilm Development. J. Bacteriol. 2003;185:4585–4592
25. Bauer J, Siala W, Tulkens PM, Van Bambeke F. A combined pharmacodynamic quantitative and qualitative model reveals the potent activity of daptomycin and delafloxacin against Staphylococcus aureus biofilms. Antimicrob. Agents Chemother. 2013;57:2726–2737
26. Whitchurch CB, Tolker-Nielsen T, Ragas, PC, Mattick, JS. Extracellular DNA Required for Bacterial Biofilm Formation. Science. 2002;295(5559):148727. Otter JA, Vickery K, Walker JT, deLancey Pulcini E, Stoodley P, Goldenberg SD, et al. Surface-attached cells, biofilms and biocide susceptibility: implications for hospital cleaning and disinfection. J Hosp Infect. 2015;89(1):16–27.
28. Percival SL, Suleman L, Vuotto C, Donelli G. Healthcare-associated infections, medical devices and biofilms: risk, tolerance and control. J Med Microbiol. 2015;64(4):323-334.
29. Devlin-Mullin A, Todd NM, Golrokhi Z, Geng H, Konerding MA, Ternan NG, et al. Atomic Layer Deposition of a Silver Nanolayer on Advanced Titanium Orthopedic Implants Inhibits Bacterial Colonization and Supports Vascularized de Novo Bone Ingrowth. Adv Healthc Mater. 2017;6(11).
30. Bayles KW. The biological role of death and lysis in biofilm development. Nature Rev. Microbiol. 2007;5:721-726


  • biocide healthcare associated pathogens biofilm Pseudomonas organosilane


Dive into the research topics of 'Evaluation of bactericidal and anti-biofilm properties of a novel surface-active organosilane biocide against healthcare associated pathogens and Pseudomonas aeruginosa biolfilm'. Together they form a unique fingerprint.

Cite this