Inhibition of pathogenic bacterial biofilms on PDMS based implants by L. acidophilus derived biosurfactant

Surekha K. Satpute, Nishigandha S. Mone, Das Parijat, Ibrahim Banat, Banpurkar Arun

Research output: Contribution to journalArticlepeer-review

25 Citations (Scopus)
31 Downloads (Pure)


Background: Lactobacillus spp. predominantly shows its presence as a normal mucosal flora of the mouth and intestine. Therefore, the objective of our research is to investigate the in-vitro conditions for the prospective of medically valuable biosurfactants (BSs) derived from Lactobacillus spp. Biosurfactant (BS) obtained from Lactobacillus spp. exhibit antibiofilm and antiadhesive activity against broad range of microbes. In the present study we investigated the production, purification and properties of key components of the cell-associated-biosurfactant (CABS) from Lactobacillus acidophilus NCIM 2903.

Results: Extracted, purified, freeze-dried CABS shows reduction in surface tension (SFT) of phosphate buffer saline (PBS @pH 7.0) from 71 to 26 mN/m and had a critical micelle concentration (CMC) of 23.6mg/mL. The CABS showed reduction in interfacial tension (IFT) against various hydrocarbons and had effective spreading capability as reflected through the decrease in contact angle (CA) on different surfaces (polydimethylsiloxane - PDMS, Teflon tape, glass surface, polystyrene film and OHP sheet). The anionic nature of CABS displayed stability at different pH and temperatures and formed stable emulsions. Thin layer chromatography (TLC) and Fourier transform infrared spectroscopy (FTIR)
revealed CABS as glycolipoprotein type. The Sodium Dodecyl Sulphate Polyacrylamide Gel Electrophoresis (SDS-PAGE) showed presence of multiple bands in a molecular range of 14.4 to 60 kDa, with prominent bands of 45 kDa. The CABS has significant antiadhesion and antibiofilm activity against tested bacterial strains.

Conclusion: The current challenging situation is to develop methods or search for the molecules that will prevent the formations of biofilm on medical bioimplants of PDMS based materials. These findings are supportive for the use of Lactobacilli derived BS as potential antiadhesive agent on various surfaces of biomedical devices.
Original languageEnglish
Article number39
Pages (from-to)1
Number of pages15
JournalBMC Microbiology
Issue number1
Publication statusPublished - 13 Feb 2019

Bibliographical note

Due to a scopus update this output was checked and the template that was originally used was conference - paper. After checking the output online the template was changed to Article so when the REF OA Assessment Section appeared it was listed as REF OA Non Compliant even though the output was validated within 3 months of the acceptance date. The article is listed online as Open Access so an exception was added accordingly.


  • Adhesion, Biosurfactant, Lactobacillus, Biofilm, Medical implants, PDMS, protein, surface tension
  • Biosurfactant
  • Medical implants, PDMS, protein, surface tension
  • Biofilm
  • Adhesion
  • Lactobacillus


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