Abstract
Lipopeptide biosurfactants (LPBs) display unique properties with widespread therapeutic applications. Recently, the wound healing activity of LPBs has received more attention. However, few investigations have focused on the healing mechanisms of these compounds, particularly those that are associated with the angiogenic pathways. Therefore, the current study aimed at
investigating the efect of a natural LPB produced by Acinetobacter. junii B6 on the angiogenic potential of human umbilical vein endothelial cells (HUVECs). The proliferation, migration, and three-dimensional tube forming potential of HUVECs were examined after treatment with LPB. By using western blotting, the expression of angiogenic-related genes such as hypoxia-inducible factor-1α (HIF-1α) and vascular endothelial growth factor (VEGF) were also measured. LPB demonstrated no signifcant proliferative or cytotoxic efect on HUVECs at the concentrations lower than 600 µg/mL. Moreover, LPB at a concentration of 300 µg/mL caused a signifcant increase in the rates of migration and tube formation of HUVECs. LPB also enhanced the protein expression levels of HIF-1α and VEGF in HUVECs in a dose-dependent manner. The present results suggest that LPB might be considered a potential wound healing agent by modifying some angiogenic factors.
investigating the efect of a natural LPB produced by Acinetobacter. junii B6 on the angiogenic potential of human umbilical vein endothelial cells (HUVECs). The proliferation, migration, and three-dimensional tube forming potential of HUVECs were examined after treatment with LPB. By using western blotting, the expression of angiogenic-related genes such as hypoxia-inducible factor-1α (HIF-1α) and vascular endothelial growth factor (VEGF) were also measured. LPB demonstrated no signifcant proliferative or cytotoxic efect on HUVECs at the concentrations lower than 600 µg/mL. Moreover, LPB at a concentration of 300 µg/mL caused a signifcant increase in the rates of migration and tube formation of HUVECs. LPB also enhanced the protein expression levels of HIF-1α and VEGF in HUVECs in a dose-dependent manner. The present results suggest that LPB might be considered a potential wound healing agent by modifying some angiogenic factors.
Original language | English |
---|---|
Number of pages | 7 |
Journal | International Journal of Peptide Research and Therapeutics |
DOIs | |
Publication status | Published - 23 Jan 2021 |
Bibliographical note
Publisher Copyright:© 2021, The Author(s), under exclusive licence to Springer Nature B.V. part of Springer Nature.
Copyright:
Copyright 2021 Elsevier B.V., All rights reserved.
Keywords
- Acinetobacter junii
- Angiogenesis
- Lipopeptide biosurfactant
- Wound healing