In the present work, a paper-based electrode assemble was developed and implemented to detect target microRNA 155 (miRNA 155) via electrochemical impedance spectroscopy (EIS) measurements. In this concept, gold nanoparticles (AuNPs) modified paper based electrode assemble system (AuNP-PE) was designed, and characterized by scanning electron microscopy (SEM), cyclic voltammetry (CV) and EIS measurements. The impedimetric detection of miRNA 155 was performed by measuring the fractional change at the charge transfer resistance (Rct). The detection limits were found as 33.8 nM in PBS and 93.4 nM in fetal bovine serum (FBS) medium, respectively. The selectivity of the proposed assay was tested against to non-complementary (NC) and mismatch (MM) miRNA sequences in the presence of mixture sample containing miRNA:NC (1:1) and miRNA: MM (1:1) in PBS (pH 7.40) or FBS. The analytical performance and the selectivity of impedimetric biosensor were also tested in FBS.
This study was supported under the Newton-Katip Celebi Funding programme and all authors acknowledge the financial support from Turkish Scientific and Technological Research Council (TUBITAK; Project no. 215Z702) and from British Council (Newton fund, Institutional Links, Ref: 216182787 ). PhD and master students respectively E.E. and E.Y. acknowledge a project scholarship through by project (TUBITAK Project no. 215Z702). Authors also acknowledge to helpful discussion of Assoc. Prof. Yildiz Uludag as the project consultant during project (TUBITAK; Project no. 215Z702). A.E. also would like to express her gratitude to the Turkish Academy of Sciences (TUBA) as a Principal member for its partial support.
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- Paper based sensor
- impedimetric sensor
- gold electrodeposition
- gold nanoparticles
- Gold nanoparticles
- Paper based electrode
- Electrochemical impedance spectroscopy