Plasma induced DNA damage: Comparison with the effects of ionizing radiation

Saša Lazović, Dejan Maletić, A Leskovac, J Filipović, Nevena Puač, Gordana Malović, Gordana Joksić, Zoran Petrović

Research output: Contribution to journalArticlepeer-review

26 Citations (Scopus)

Abstract

We use human primary fibroblasts for comparing plasma and gamma rays induced DNA damage. In both cases, DNA strand breaks occur, but of fundamentally different nature. Unlike gamma exposure, contact with plasma predominantly leads to single strand breaks and base-damages, while double strand breaks are mainly consequence of the cell repair mechanisms. Different cell signaling mechanisms are detected confirming this (ataxia telangiectasia mutated - ATM and ataxia telangiectasia and Rad3 related - ATR, respectively). The effective plasma doses can be tuned to match the typical therapeutic doses of 2 Gy. Tailoring the effective dose through plasma power and duration of the treatment enables safety precautions mainly by inducing apoptosis and consequently reduced frequency of micronuclei.
Original languageEnglish
Article number 124101
Number of pages5
JournalApplied Physics Letters
Volume105
Issue number12
DOIs
Publication statusPublished - 24 Sep 2014

Keywords

  • atmospheric pressure plasmas
  • DNA damage
  • apoptosis
  • single strand breaks
  • double strand breaks
  • gamma rays
  • gamma ray treatment

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